Passive Hemagglutination-Inhibition Test for Typing Foot-and-Mouth Disease Virus1

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Passive hemagglutination test for bovine chlamydial abortion.

Purified chlamydial elementary bodies were subjected to sonic lysis to produce soluble antigen for passive hemagglutination (PHA). PHA was more sensitive than complement fixation (CF) in detecting antibody in hyperimmunized rabbits, lambs, and calves. Both CF and PHA tests were used to monitor antibody responses in experimentally infected pregnant cows. All animals responded with rises in titer...

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Detection of foot-and-mouth disease virus antibodies. II. Use of fractionated bovine antisera for improving the specificity of a "passive" hemagglutination test.

Because 7S immunoglobulin (Ig) G antibodies of low type specificity were present in mixtures with highly specific 19S IgM antibodies, many bovine antisera to foot-and-mouth disease virus (FMDV) type A(12), strain 119 cross-reacted with type O of FMDV and to some degree with type C in the passive hemagglutination (HA) test. After 19S IgM antibodies were separated by density gradient centrifugati...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1972

ISSN: 0003-6919

DOI: 10.1128/aem.23.1.98-103.1972